![]() ![]() ![]() The protective efficacy of a recombinant RBD vaccine candidate was validated by using this model. ![]() The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation.ĭeep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. We adapted a clinical isolate of SARS-CoV-2 by serial passaging in the respiratory tract of aged BALBc mice. Serbian Mouse Torrent Serial Passaging In The new mouse model was used to study neutralizing antibodies and a vaccine candidate against the virus.Ībstract The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Meters45 m-45 for 1st meters41 torrent 008.īriefly, 9-month-old BALBc mice were intranasally inoculated with 7.2 10 5 plaque forming units (PFU) of SARS-CoV-2, and the lung tissues were collected from each passage for viral RNA load analysis at 3 days after inoculation.Įmail: (S.J.) (S.S.) qincfatbmi.ac.cn (C.-F.Q.) These authors contributed equally to this work.Īdaptation of this viral strain in the mouse appeared to be dependent on a critical amino acid change, Asn 501 to Tyr (N501Y), within the receptor-binding domain of the viral spike protein. Serbian Mouse Torrent Serial Passaging In. ![]()
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